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Article
Composition of Amesiodendron chinense (Merr.) Hu Seed Oil and Assessment of Its Nrf2/ARE Induction Activity in AREc32 Cells
Chuanchom Khuniad1,2,*, Lutfun Nahar 3,*, Jason W. Birkett 4, Kenneth J. Ritchie 1 and Satyajit D. Sarker 1,*
1 Centre for Natural Products Discovery (CNPD), School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, Liverpool L3 3AF, UK
2 Department of Thai Traditional Medicine, Faculty of Health and Sports Science, Thaksin University, Phatthalung 93210, Thailand
3 Laboratory of Growth Regulators, Palacký University and Institute of Experimental Botany, The Czech Academy of Sciences, Šlechtitelů 27, 78371 Olomouc, Czech Republic
4 Forensic Science Research Group, School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, Liverpool L3 3AF, UK
* Correspondence: C.Khuniad@2020.ljmu.ac.uk (C.K.); nahar@ueb.cas.cz (L.N.) and S.Sarker@ljmu.ac.uk (S.D.S.)
Received: 7 April 2024; Revised: 26 April 2024; Accepted: 6 May 2024; Published: 14 May 2024
Abstract: Background: Amesiodendron chinense (Merr.) Hu (family: Sapindaceae) is a Thai medicinal plant. The seed oil of this species has been used by folk healers and local people in southern Thailand for the treatment of wounds, skin disorders and common hair problems. This study aimed at the GC-MS-based determination of the chemical composition of the seed oil of this plant, and evaluation of its Nrf2/ARE induction activity in AREc32 cells (modified human breast cancer cell line MCF-7) using the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] and luciferase reporter gene assays. Results: GC-MS analysis identified 9-(E)-octadecenoic acid (84.82%) as the main component of this seed oil. TLC-based qualitative DPPH (2,2-diphenyl-1-picrylhydrazyl) assay revealed the DPPH radical-scavenging activity of the seed oil and its chromatographic fractions. A low-level DPPH-scavenging activity was observed in the quantitative assay, but no IC50 value could be determined even with the highest tested concentration (10 mg/mL). Neither the oil nor its chromatographic fractions showed any significant Nrf2/ARE induction in AREc32 cells. The seed oil was noncytotoxic against the AREc32 cells. Conclusions: A. chinense seed oil and its fractions had a low level of free-radical scavenging property but no significant Nrf2/ARE induction activity in AREc32 cells. However, as the oil did not show any cytotoxicity at test concentrations in the MTT assay, this oil might potentially be safe to use in cosmetic formulations or as a vehicle for the dermal delivery of drug molecules.
Keywords:
Amesiodendron chinense Sapindaceae seed oil antioxidant cytotoxicity cancer chemoprevention GC-MS Nrf2/ARE inductionReferences
- Convention on Biological Diversity, Thailand - Main Details Biodiversity Facts. Available online: https://www.cbd.int/countries/profile/?country=th (accessed on 2 July 2023).
- Chokevivat, V.; Chuthaputti, A. The role of Thai traditional medicine in health promotion. Proceedings of the 6th Global Conference on Health Promotion. Bangkok, Thailand, on 7-11 August 2005. Citeseer: Princeton, NJ, USA, 2005, 1‒25.
- Kanjanahattakij, N.; Kwankhao, P.; Vathesatogkit, P.; et al. Herbal or traditional medicine consumption in a Thai worker population: pattern of use and therapeutic control in chronic diseases. BMC Complement Altern. Med. 2019, 19, 258.
- Ban, H.V.; Van, T.T.T.; Chien, V.V.; et al. Flavone C-glycosides from the leaves of Amesiodendron chinense. Phytochem. Lett. 2020, 40, 105‒108.
- National Parks Board of Singapore, Amesiodendron chinense (Merrill) Hu. Available online: https://www.nparks.gov.sg/FloraFaunaWeb/Flora/2/7/2707 (accessed on 2 July 2023).
- Sampantamit, T.; Katemai, W. Utilization and chemical composition of Khun oil in tambon Klongchalern and tambon Charad, Knog-hra district, Phatthalung province. Available online: http://kb.tsu.ac.th/jspui/bitstream/123456789/1924/1/%E0%B8%97%E0%B8%B4%E0%B8%9E%E0%B8%A2%E0%B9%8C%E0%B8%97%E0%B8%B4%E0%B8%A7%E0%B8%B2%20%E0%B8%AA%E0%B8%B1%E0%B8%A1%E0%B8%9E%E0%B8%B1%E0%B8%99%E0%B8%98%E0%B8%A1%E0%B8%B4%E0%B8%95%E0%B8%A3%2000153185.pdf (accessed on 7 April 2024)
- World Conservation Monitoring Centre, Amesiodendron chinense. The IUCN Red List of Threatened Species 1998. Available online: https://www.iucnredlist.org/species/35893/9960183 (accessed on 2 July 2023).
- Upho, U. (2005) Ethnobotany of Buddhist and Muslim Thais in some locations in the lower part of southern Thailand. PhD thesis, Chiangmai University, Chiangmai, Thailand, 30 September 2005.
- Wangpradit, N.; Macha, S.; Phooteh, N.; et al. Determination of required hydrophilic-lipophilic balance of Amesiodendron chinense (Merr.) Hu oil and development of stable cream formulation. Lipids Cosmet. 2022, 29, 2022011.
- Ban, H.V.; Van, T.T.T.; Chien, V.V.; et al. Lignans from leaves of Amesiodendron chinense and their cytotoxic activity. Vietnam J. Sci. Technol. 2020, 58, 442‒449.
- Ban, H.V.; Van, T.T.T.; Chien, V.V.; et al. Flavonoids from flowers of Amesiodendron chinense. Vietnam J. Sci. Technol. 2020, 58, 676‒684.
- Reid, R.G.; Sarker, S.D. Isolation of natural products by low-pressure column chromatography. In Natural Products Isolation, 3rd ed.; Sarker, S. D. and Nahar, L. (ed.); Humana Press: Totowa, NJ, USA, 2012, pp. 155‒188.
- Takao, T.; Kitatani, F.; Watanabe, N.; et al. A simple screening method for antioxidants and isolation of several antioxidants produced by marine bacteria from fish and shellfish. Biosci. Biotechnol. Biochem. 1994, 58, 1780‒1783.
- Chima, N.K.; Nahar, L.; Majinda, R.R.T.; et al. Assessment of free-radical scavenging activity of Gypsophila pilulifera: assay-guided isolation of verbascoside as the main active component. Rev. Bras. Farmacogn. 2014, 24, 38‒43.
- Kumarasamy, Y.; Byres, M.; Cox, P.J.; et al Screening seeds of some Scottish plants for free radical scavenging activity. Phytother. Res. 2007, 21, 615‒621.
- Basar, N.; Nahar, L.; Oridupa, O.A.; et al. Utilization of the ability to induce activation of the nuclear factor (erythroid-derived 2)-like factor 2 (Nrf2) to assess potential cancer chemopreventive activity of liquorice samples. Phytochem. Anal. 2016, 27, 233‒238.
- Mosmann, T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods 1983, 65, 55−63.
- Uddin, G.; Rauf, A.; Gull, S.; et al. Proximate chemical composition and biological profile of fatty acids of Withania somnifera L dunal. J. Med. Plants Res. 2013, 27, 2034−2039.
- Low, J.N.; Scrimgeour, C.; Horton, P. Elaidic acid (trans-9-octadecenoic acid). Crystallogr. Commun. 2005, 61, o3730−o3732.
- Nielsen, L.V.; Krogager, T.P.; Young, C.; et al. Effects of elaidic acid on lipid metabolism in HepG2 cells, investigated by an integrated approach of lipidomics, transcriptomics and proteomics. PloS one 2013, 8, e74283.
- Abbey, M.; Nestel, P.J. Plasma cholesteryl ester transfer protein activity is increased when trans-elaidic acid is substituted for cis-oleic acid in the diet. Atherosclerosis 1994, 106, 99−107.
- Frantianni, F.; d’Acierno, A.; Ombra, M.N.; et al. Fatty acid composition, antioxidant and in vitro anti-inflammatory activity of five cold-pressed Prunus seed oils and their anti-biofilm effect against pathogenic bacteria. Front. Nutr. 2021, 8, 775751.
- Ranjan, A.; Ramachandran, S.; Gupta, N.; et al. Role of phytochemicals in cancer prevention. Int. J. Mol. Sci. 2019, 20, 4981.
- Alvarez, A.M.; Rodriguez, M.L.G. Lipids in pharmaceutical and cosmetic preparations. Grasas Aceites 2000, 52, 74‒96.